Mars One plans to send four astronauts to the red planet in 2030, with additional crews of four arriving every two years.
According to the organization, 1,000 volunteers have already signed up for the one-way trip.
The project is being funded by corporate and personal donations.
A "large group" of ambassadors - including an astronaut, a physicist and NASA scientists - are also involved.
"Sending humans to Mars has been my dream for twenty years. Evidently, I am not alone -- we have received emails from over fifty countries ... regardless of their background, people are positive about this optimistic event that we believe will bring people of Earth a little bit closer together," said Arno Wielders, co-founder and technical director of Mars One, in a statement.
Mars One will begin selecting its astronauts in early 2013.
The organization plans to detail "every aspect" of the mission through a reality TV show.